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KMID : 1189420220350030036
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Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology
2022 Volume.35 No. 3 p.36 ~ p.47
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Research of the Anti-inflammatory Effects of Hwadokdan on Raw 264.7 Cells
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Shin Ki-Seok
Hwangbo Min
Jee Seon-Young
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Abstract
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Objectives : The purpose of this study was to evaluate the anti-Inflammatory effect of Hwadokdan(HDD) extracted with water.
Methods : We pretreated LPS-activated Raw 264.7 cell with HDD and anti-inflammatory effect of HDD is investigated by measuring NO production, relative iNOS, and TNF-α, IL-6, IL-1β, PGE2 productions level, p-IκBα, IκBα, NFκB.
Results : HDD reduced NO production and iNOS protein, TNF-α, IL-6 level at concentration of 100㎍/㎖ and 300㎍/㎖ in LPS-activated Raw 264.7 cell significantly. There wasn't a significant cytotoxicity in MTT assay. And HDD reduced IL-1β level at concentration of 300㎍/㎖ in LPS-activated Raw 264.7 cell significantly.
HDD reduced p-IκBα and NFκB protein level at concentration of 300㎍/㎖ in LPS-activated Raw 264.7 cell significantly. And HDD enhanced IκBα protein level at concentration of 100㎍/㎖ and 300㎍/㎖ in LPS-activated Raw 264.7 cell significantly.
Conclusions : These suggests that HDD can be used as a therapeutic drug for various inflammatory diseases.
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KEYWORD
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Hwadokdan(HDD), Lonicerae Flos, Prunellae Spica, Inflammation, NFκB.
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